Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Galanin Inhibits Acetylcholine Release in the Ventral Hippocampus of the Rat: Histochemical, Autoradiographic, in vivo, and in vitro Studies
Beteiligte:
Fisone, Gilberto;
Wu, Chun F.;
Consolo, Silvana;
Nordström, Öie;
Brynne, Niclas;
Bartfai, Tamas;
Melander, Tor;
Hökfelt, Tomas
Erschienen:
National Academy of Sciences of the United States of America, 1987
Erschienen in:
Proceedings of the National Academy of Sciences of the United States of America, 84 (1987) 20, Seite 7339-7343
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
<p>A high density of galanin binding sites was found by using <sup>125</sup>I-labeled galanin, iodinated by chloramine-T, followed by autoradiography in the ventral, but not in the dorsal, hippocampus of the rat. Lesions of the fimbria and of the septum caused disappearance of a major population of these binding sites, suggesting that a large proportion of them is localized on cholinergic nerve terminals of septal afferents. As a functional correlate to these putative galanin receptor sites, it was shown, both in vivo and in vitro, that galanin, in a concentration-dependent manner, inhibited the evoked release of acetylcholine in the ventral, but not in the dorsal, hippocampus. Intracerebroventricularly applied galanin (10 μ g/15 μ l) fully inhibited the scopolamine (0.5 mg/kg, s.c.)-stimulated release of acetylcholine in the ventral, but not in the dorsal, hippocampus, as measured by microdialysis technique. In vitro, galanin inhibited the 25 mM K<sup>+</sup>-evoked release of [<sup>3</sup>H]acetylcholine from slices of the ventral hippocampus, with an IC<sub>50</sub> value of ≈ 50 nM. These results are discussed with respect to the colocalization of galanin- and choline acetyltransferase-like immunoreactivity in septal somata projecting to the hippocampus.</p>