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Medientyp:
E-Artikel
Titel:
Expression and Nucleotide Sequence of a Plasmid-Determined Divalent Cation Efflux System from Alcaligenes eutrophus
Beteiligte:
Nies, Dietrich H.;
Nies, Anke;
Chu, Lien;
Silver, Simon
Erschienen:
National Academy of Sciences of the United States of America, 1989
Erschienen in:
Proceedings of the National Academy of Sciences of the United States of America, 86 (1989) 19, Seite 7351-7355
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
Resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pMOL30 in Alcaligenes eutrophus results from a cation efflux system. Five membrane-bound polypeptides that were expressed in Escherichia coli from this determinant under the control of a phage T7 promoter were assigned to four open reading frames identified in the nucleotide sequence of the 6881-base-pair fragment containing the czc putative operon. The contributions of the polypeptides to the cation efflux system were analyzed with deletion derivatives of the 6.9-kilobase fragment, constructed, and expressed in E. coli under the control of the phage T7 promoter and in A. eutrophus under the control of the lac promoter.