Beschreibung:
<p> Plant regeneration of maize genotypes known to be resistant to leaf-feeding by the fall armyworm, Spodoptera frugiperda (J. E. Smith), was attempted via somatic embryogenesis. Successful regeneration from callus tissue cultures was achieved for the maize genotypes 'Antigua 2D-118', 'MpSWCB-4', 'Mp496', and some selected single crosses. Progeny from these regenerates were evaluated under laboratory and field conditions for their levels of resistance to fall armyworm leaf-feeding as compared to their respective non-regenerated parental lines. Significant differences in resistance to leaf-feeding by the fall armyworm were observed between the regenerated and non-regenerated lines. Differences were of a positive as well as a negative nature in regards to the levels of resistance in the regenerates as compared to their non-regenerated parental line. This is further evidence of the potential of somaclonal variation for obtaining agronomically useful traits for crop improvement, in this instance plant resistance to insect attack. /// Se intentó, por medio de embriogenenesis somaticas, la regeneración de plantas de genotipos de maíz resistentes a el consumo de hojas del cogollero Spodoptera frugiperda (J. E. Smith). La regeneración de callos de cultivos de tejido fue lograda para los genotipos "Antigua2D-118," "MpSWCB-4," Mp496, y para algunos cruces simples. Bajo condiciones de laboratorio e invernadero, se compararon los niveles de resistencia a el cogollero entre la progenie obtenida a partir de plantas regeneradas y comparada con las lineas no regeneradas de los padres. Las diferencias fueron de naturaleza positivas y negativa en referencia a los niveles de resistencia en las lineas regeneradas y comparados con sus lineas paternas no regeneradas. Esto se considera como una evidencia mas del potencial de la variacion somaclonal para obtener caracteres de mejoramiento del cultivo para resistencia de este a el ataque de insectos. </p>