Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Radiosensitivity of Cultured Insect Cells: I. Lepidoptera
Beteiligte:
Koval, Thomas M.
Erschienen:
Academic Press, Inc., 1983
Erschienen in:
Radiation Research, 96 (1983) 1, Seite 118-126
Sprache:
Englisch
ISSN:
0033-7587;
1938-5404
Entstehung:
Anmerkungen:
Beschreibung:
<p>The radiosensitivity of five lepidopteran insect cell lines representing five different genera has been investigated. These lines are: (1) TN-368, Trichoplusia ni; (2) IPLB-SF-1254, Spodoptera frugiperda; (3) IPLB-1075, Heliothis zea; (4) MRRL-CH1, clone GV1, Manduca sexta; and (5) IAL-PID2, Plodia interpunctella. The cell lines grew at different rates and had population doubling times that ranged from 19 to 52 hr. All of the lines are highly heteroploid and have approximate chromosome numbers near or above 100. The chromosomes are very small. All of the lines are extremely radioresistant; cell populations are able to recover from 260 kVp X-ray exposures up to and including 400 Gy, the highest dose examined. Cell survival curves were obtainable for only the TN-368 and IPLB-SF-1254 lines. The TN-368 cells displayed a biphasic survival response with <tex-math>$D_{0},\ D_{{\rm q}}$</tex-math>, and n values of 65.7 and 130.2 Gy, 9.0 and -36.1 Gy, and 1.2 and 0.8, respectively, for the steep and shallow portions of the curve. The IPLB-SF-1254 cells had a D<sub>0</sub> of 63.9 Gy, <tex-math>$D_{{\rm q}}$</tex-math> of 19.0 Gy, and n value of 1.4. These studies provide definitive evidence of the radioresistance of lepidopteran cells, and suggest that this radioresistance is a characteristic of lepidopteran insects.</p>