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Medientyp:
E-Artikel
Titel:
Gamma-Ray- and UV-Sensitive Strains of a Radioresistant Cell Line: Isolation and Cross-Sensitivity to Other Agents
Beteiligte:
Koval, Thomas M.
Erschienen:
Academic Press, Inc., 1991
Erschienen in:
Radiation Research, 127 (1991) 1, Seite 58-63
Sprache:
Englisch
ISSN:
0033-7587;
1938-5404
Entstehung:
Anmerkungen:
Beschreibung:
<p>Two γ-ray-sensitive and two ultraviolet (UV)-sensitive variants were isolated from the γ-ray- and UV-resistant TN-368 lepidopteran insect cell line. The isolation was performed by inducing mutations in the TN-368 cells using ethyl methanesulfonate, growing them for an expression period, irradiating with <sup>137</sup> Cs γ rays or 254-nm UV radiation, allowing cells to incorporate 5-bromodeoxyuridine (BrdU) in the presence of hydroxyurea (DNA repair synthesis), and finally irradiating with 365-nm UV radiation to cause DNA strand breakage at sites of BrdU incorporation with the intent of killing those cells that have undergone DNA repair synthesis and sparing those cells which, for a variety of reasons, did not. The survival of the Cs2 and Cs7 variants exposed to X rays is significantly different from the parent TN-368 line at the P < 0.0001 level. The survival of the UV10 and UV19 variants exposed to UV radiation is different from the parent at the P < 0.0001 and P < 0.003 levels, respectively. In cross-sensitivity testing of the γ-ray-sensitive variants, only Cs2 is more sensitive to 254-nm UV and only Cs7 is more sensitive to 44°C heating; both are sensitive to PUVA. The UV-sensitive mutants are both sensitive to X irradiation, PUVA, and mitomycin C. However, UV10 is not sensitive to 44°C heating while UV19 is, making UV19 the only variant strain sensitive to all agents examined. Despite the isolation procedure which was intended to select for DNA repair-deficient cells, the results suggest that a more general mechanism is responsible for the sensitivity of the variant cells to the agents tested.</p>