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Medientyp:
E-Artikel
Titel:
Chloroplast Ribosomal Protein S7 of Chlamydomonas Binds to Chloroplast mRNA Leader Sequences and May Be Involved in Translation Initiation
Beteiligte:
Fargo, David C.;
Boynton, John E.;
Gillham, Nicholas W.
Erschienen:
American Society of Plant Physiologists, 2001
Erschienen in:
The Plant Cell, 13 (2001) 1, Seite 207-218
Sprache:
Englisch
ISSN:
1040-4651
Entstehung:
Anmerkungen:
Beschreibung:
Certain mutations isolated in the 5′ untranslated region (5′UTR) of the chloroplast rps7 gene in Chlamydomonas reduce expression of reporter genes. Second site suppressors in this 5′UTR sequence restore reporter expression. 5′UTR sequences with the original mutations fail to bind a 20-kD protein, one of five proteins that bind to leaders of several chloroplast genes. However, 5′UTRs from suppressed mutants restore binding to this protein but do not bind a 47-kD protein present on the wild type and the original mutant 5′UTRs. The 20-kD protein was shown to be the S7 protein of the chloroplast ribosomal small subunit encoded by rps7, whereas the 47-kD protein was shown to be RB47, a poly(A) binding protein. Our data are consistent with the hypothesis that the S7 protein plays either a general or a specific regulatory role in translation initiation in the chloroplast.