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Media type:
Book
Title:
Enzyme engineering
:
methods and protocols
Contains:
Tripartite fusion system for the selection of protein variants with increased stability in vivo
/ Linda Foit and James C.A. Bardwell
Determining enzyme kinetics via isothermal titration calorimetry
/ Neil A. Demarse ... [et al.]
GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria
/ Olga Paley ... [et al.]
Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties
/ Sarah K. Baxter ... [et al.]
TAL effector nuclease (TALEN) engineering
/ Ting Li and Bing Yang
In vitro evolution of enzymes
/ Misha V. Golynskiy ... [et al.]
Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo
/ Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington
Reconstructing evolutionary adaptive paths for protein engineering
/ Megan F. Cole ... [et al.]
Oligonucleotide recombination enabled site-specific mutagenesis in bacteria
/ Bryan M. Swingle
FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants
/ Eric R. Geertsma
Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes
/ Hidehiko Hirakawa and Teruyuki Nagamune
Gene synthesis by assembly of deoxyuridine-containing oligonucleotides
/ Romualdas Vaisvila and Jurate Bitinaite
Protein engineering : single or multiple site-directed mutagenesis
/ Pei-Chung Hsieh and Romualdas Vaisvila
Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination
/ Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish
Promiscuity-based enzyme selection for rational directed evolution experiments
/ Sandeep Chakraborty ... [et al.]
Rational protein sequence diversification by multi-codon scanning mutagenesis
/ Jia Liu and T. Ashton Cropp
Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter
/ Jian-Wei Liu and David L. Ollis
In vitro directed evolution of enzymes expressed by E. coli in microtiter plates
/ Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis.