• Medientyp: Buch
  • Titel: Enzyme engineering : methods and protocols
  • Enthält: Tripartite fusion system for the selection of protein variants with increased stability in vivo / Linda Foit and James C.A. Bardwell
    Determining enzyme kinetics via isothermal titration calorimetry / Neil A. Demarse ... [et al.]
    GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria / Olga Paley ... [et al.]
    Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties / Sarah K. Baxter ... [et al.]
    TAL effector nuclease (TALEN) engineering / Ting Li and Bing Yang
    In vitro evolution of enzymes / Misha V. Golynskiy ... [et al.]
    Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo / Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington
    Reconstructing evolutionary adaptive paths for protein engineering / Megan F. Cole ... [et al.]
    Oligonucleotide recombination enabled site-specific mutagenesis in bacteria / Bryan M. Swingle
    FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants / Eric R. Geertsma
    Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes / Hidehiko Hirakawa and Teruyuki Nagamune
    Gene synthesis by assembly of deoxyuridine-containing oligonucleotides / Romualdas Vaisvila and Jurate Bitinaite
    Protein engineering : single or multiple site-directed mutagenesis / Pei-Chung Hsieh and Romualdas Vaisvila
    Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination / Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish
    Promiscuity-based enzyme selection for rational directed evolution experiments / Sandeep Chakraborty ... [et al.]
    Rational protein sequence diversification by multi-codon scanning mutagenesis / Jia Liu and T. Ashton Cropp
    Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter / Jian-Wei Liu and David L. Ollis
    In vitro directed evolution of enzymes expressed by E. coli in microtiter plates / Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis.
  • Beteiligte: Samuelson, James C. [Sonstige Person, Familie und Körperschaft]
  • Erschienen: New York, N.Y.; Heidelberg [u.a.]: Humana Press, 2013
  • Erschienen in: Methods in molecular biology ; 978
    Springer protocols
  • Umfang: XI, 252 S.; graph. Darst
  • Sprache: Englisch
  • ISBN: 9781627032926
  • RVK-Notation: WC 4350 : Enzymatische Analyse, Methoden der Enzymologie (auch: ELISA)
    WC 4150 : Allgemeines, Praktika; "Methods of biochemical analysis"
  • Schlagwörter: Enzym > Biotechnologie
    Enzym > Gentechnologie
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  • Signatur: WC 4150 W181-978
  • Barcode: 33117388