Media type: Text; E-Article Title: Bioorthogonal metal-free click-ligation of cRGD-pentapeptide to alginate Contributor: Krause, Andreas [Author]; Kirschning, Andreas [Author]; Dräger, Gerald [Author] Published: Cambridge : Royal Society of Chemistry, 2012 Published in: Organic and Biomolecular Chemistry 10 (2012), Nr. 29 Issue: published Version Language: English DOI: https://doi.org/10.15488/2159; https://doi.org/10.1039/c2ob25604e ISSN: 1477-0520 Keywords: Hexuronic Acids ; hexuronic acid ; glucuronic acid ; 1 ,3-dipolar cycloadditions ; Polymers ; Potential applications ; copper ; polymer ; Biomedical applications ; cyclic arginine-glycine-aspartic acid peptide ; Cycloadditions ; Medical applications ; Cyclic ; cyclopeptide ; Cycloaddition ; Reaction progress ; Peptides ; Biomacromolecule ; Alginates ; Bioactive polymers ; chemistry ; ligation ; Conjugation process ; click chemistry ; [...] Origination: Footnote: Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen. Description: Click reactions have become very common and powerful ligation techniques, of which 1,3-dipolar cycloadditions have most frequently been employed. Since metal-mediated cycloadditions are incompatible in biomedical applications due to toxicity issues associated with transition metals like copper, metal-free variants provide important alternatives. The metal-free conjugation process is studied in detail with special emphasis put on the reaction progress. This report unfolds the first aqueous metal-free "click" conjugation of a cyclic RGD-pentapeptide with the biomacromolecule alginate, creating a "smart" bioactive polymer with potential applications in biomedicine. © 2012 The Royal Society of Chemistry. Access State: Open Access