Published:
National Academy of Sciences of the United States of America, 1983
Published in:
Proceedings of the National Academy of Sciences of the United States of America, 80 (1983) 15, Seite 4659-4663
Language:
English
ISSN:
0027-8424
Origination:
Footnote:
Description:
Active DNA helicase I (Mr180,000) can be isolated from Escherichia coli F+strains but not F-strains. The transfer of the F sex factor to F-strains by conjugation permits the purification of the enzyme from the transconjugant strains. We conclude from this that helicase I is coded for by a portion of the F factor. Results also obtained by using recombinant plasmids carrying different DNA fragments of the F factor transfer region suggest that DNA helicase I is identical to the product of traI, one of the transfer genes of the F factor.