Erschienen:
National Academy of Sciences of the United States of America, 1983
Erschienen in:
Proceedings of the National Academy of Sciences of the United States of America, 80 (1983) 15, Seite 4659-4663
Sprache:
Englisch
ISSN:
0027-8424
Entstehung:
Anmerkungen:
Beschreibung:
<p>Active DNA helicase I (M<sub>r</sub>180,000) can be isolated from Escherichia coli F<sup>+</sup>strains but not F<sup>-</sup>strains. The transfer of the F sex factor to F<sup>-</sup>strains by conjugation permits the purification of the enzyme from the transconjugant strains. We conclude from this that helicase I is coded for by a portion of the F factor. Results also obtained by using recombinant plasmids carrying different DNA fragments of the F factor transfer region suggest that DNA helicase I is identical to the product of traI, one of the transfer genes of the F factor.</p>