Isobe, Masaharu;
Russo, Giandomenico;
Haluska, Frank G.;
Croce, Carlo M.
Cloning of the Gene Encoding the δ Subunit of the Human T-Cell Receptor Reveals Its Physical Organization within the α -Subunit Locus and Its Involvement in Chromosome Translocations in T-Cell Malignancy
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Media type:
E-Article
Title:
Cloning of the Gene Encoding the δ Subunit of the Human T-Cell Receptor Reveals Its Physical Organization within the α -Subunit Locus and Its Involvement in Chromosome Translocations in T-Cell Malignancy
Contributor:
Isobe, Masaharu;
Russo, Giandomenico;
Haluska, Frank G.;
Croce, Carlo M.
Published:
National Academy of Sciences of the United States of America, 1988
Published in:
Proceedings of the National Academy of Sciences of the United States of America, 85 (1988) 11, Seite 3933-3937
Language:
English
ISSN:
0027-8424
Origination:
Footnote:
Description:
<p>By taking advantage of ``chromosomal walking'' techniques, we have obtained clones that encompass the T-cell receptor (TCR) δ -chain gene. We analyzed clones spanning the entire J<sub>α</sub> region extending 115 kilobases 5<sup>′</sup> of the TCR α -chain constant region and have shown that the TCR δ -chain gene is located over 80 kilobases 5<sup>′</sup> of C<sub>α</sub>. TCR δ -chain gene is rearranged in the γ /δ -expressing T-cell line Peer and is deleted in α /β -expressing T-cell lines. Sequence analysis of portions of this genomic region demonstrates its identity with previously described cDNA clones corresponding to the C<sub>δ</sub> and J<sub>δ</sub> segments. Furthermore, we have analyzed a t(8;14)(q24;q11) chromosome translocation from a T-cell leukemia and have shown that the J<sub>δ</sub> segment is rearranged in cells deriving from this tumor and probably directly involved in the translocation. Thus, the newly cloned TCR δ chain is implicated in the genesis of chromosome translocations in T-cell malignancies carrying cytogenetic abnormalities of band 14q11.</p>